Objective        To determine the effect of silencing phospholipase Cε (PLCε) gene by RNA interference on the nuclear translocation of p65 and cell proliferation in human 786-0 renal cancer cells.       Methods        A PLCε specific shRNA expression vector pGenesil-PLCε was transfected into 786-0 cells by Lipofectamine 2000. Colony cell survival assay were used to determine effects on cellular proliferation ability after transfection. The expression of PLCε

c-Myc and COX-2 was detected by RT-PCR. The expression of PLCε

c-Myc

COX-2

total p65 and p65 in the nucleus were detected by Western blotting. Immunofluorescence assay was used to detect the location of p65. The expression of c-Myc and COX-2 was also measured by Western blotting after BAY11-7082

an inhibitor of p65

treatment with different doses for different time periods.       Results        786-0 cells proliferation was inhibited after PLCε shRNA treatment in vitro. In 786-0 renal cancer cells

the expression of PLCε

transcription factor p65 in the nucleus and its downstream gene c-Myc and COX-2 was decreased using PLCε specific shRNA (P0.01). Immunofluorescence assay results showed that p65 translocated to the nucleus was suppressed after  PLCε shRNA transfection. The expression of c-Myc and COX-2 at protein level were inhibited by BAY11-7082 in a dose- and time-dependent manner (P0.01).       Conclusion        Silencing PLCε inhibits 786-0 cell proliferation via inhibiting nuclear translocation of p65 and expression of c-Myc and COX-2.