Objective      To explore the effect of mitomycin C on laryngotracheal stenosis in rabbits.       Methods      Rabbits were randomly divided into 5 groups

that is

  a control group (group A)

a model group (group B)

a 0.5 mg/mL low-dose group (group C1)

a 2 mg/mL middle-dose group (group C2) and a 5 mg/mL high-dose group (group C3). The model of rabbit laryngotracheal stenosis was established by scraping the laryngotracheal mucosa. All rabbits in group C1

group C2 and group C3 received topical application of mitomycin C to the wound surface of larygotracheal mucosa. The laryngotracheal stenosis degree was observed on the 20th day after operation. The fibroblasts taken from the stenosis tissue of the model group were cultured in vitro with mitomycin C at 6 different concentrations (0

2.5

12.5

50

100 mg/mL

and 200 mg/mL). Meanwhile

the fibroblast apoptosis difference was detected by flow cytometry. In addition

the mRNA and protein expression of basic fibroblast growth factor (bFGF) and transforming growth factor β1 (TGF-β1) in the 6 groups was detected by FQ-PCR and Western blotting

respectively.       Results      Compared with group A

both group B and group C showed obvious laryngotracheal stenosis (P0.05). The stenosis degree of group C2 and group C3 was significantly lower than that of group B (P0.05)

but there was no significant difference between group B and group C1 (P0.05). Mitomycin C could significantly inhibit the proliferation of the fibroblasts and reduce the mRNA and protein expression of TGF-β1 and bFGF.       Conclusion      Mitomycin C can significantly induce the apoptosis of fibroblasts

and may play an important role in preventing and treating laryngotracheal stenosis.