Objective      To investigate the effects of protocadherin 10 (PCDH10) gene on proliferation

migration and invasion of multiple myeloma (MM) cell line RPMI-8226 and its mechanism.       Methods      The RPMI-8226 cell line was transfected with pcDNA3.1(+)PCDH10 and pcDNA3.1(+) by lipofectamine and named as PCDH10 transfected group and empty vector group

respectively

with untreated cells as control. Real-time PCR and Western blot was used to investigate mRNA and protein expression of PCDH10. MTT assay was employed to determine cell proliferation. Transwell chamber and Matrigel assay were applied to observe the ability of migration and invasion. Western blotting was used to detect the expression of related proteins.       Results      The levels of mRNA and protein of PCDH10 were highly expressed in the PCDH10 transfected group. MTT assay showed that the proliferation of PCDH10-8226 cells was suppressed in the PCDH10 transfected group. The numbers of migration and invasion cells the PCDH10 transfected group were 51.00±2.65 and 51.00±8.00

respectively

significantly less than those from the control group (P0.05). Western blot results showed that PCDH10 could down-regulate the expressions of AKT

p-AKT

cyclinD1 and MMP-9 (P0.05).       Conclusion      PCDH10 gene inhibits proliferation

migration and invasion in MM cell line RPMI-8226

which may involves PI3K/ATK pathway.