Objective       To determine the killing effect of high-intensity focused ultrasound (HIFU) induced albendazole thermosensitive liposomes (TSL-ABZ) on protoscolices in vitro.       Methods       Film dispersion was used to prepare albendazole thermosensitive liposomes. The experiment was carried out on 8 different groups

that is

the sham ultrasound group

simple HIFU group

HIFU+ABZ agent 100 μL group and HIFU+TSL-ABZ 100

200

300

600 and 1 000 μL groups. In each group

1 mL suspension of protoscolices was added into 1 mL PBS buffer mixed with different doses of liposomes

and then exposed to HIFU irradiation of 75 W for 12 s. Eosin staining was carried out after HIFU treatment. Light microscopy was used to observe the morphological change and the instant death rates of protoscolices. HE staining was made after the suspension was fasted with 4% paraformaldehyde.       Results       The prepared TSL-ABZ liposomes were in regular shape and well dispersed

and showed better killing effect on protoscolices when combined with HIFU. HIFU+TSL-ABZ 1 000 μL resulted in best killing efficiency [（88.25±0.01）%，P0.01]. Morphological observation showed that heavily damaged membrane

disappeared calcium particles

obvious vacuolation

and fell-off hook and some structures even missing.       Conclusion       HIFU induced TSL-ABZ enhances the killing effect of HIFU on protoscolices. When the power of HIFU is constant and the dose of TSL-ABZ is within a certain range

the mortality of protoscolices is increased in a dose-dependent manner.