Objective      To observe the in vitro induction successful rate and stability of fluconazole-resistant Trichosporon asahii (T. asahii) with fluconazole combined with methylprednisolone.       Methods      Sixteen strains of T. asahii underwent in vitro drug susceptibility test. The strains were induced with fluconazole alone

fluconazole combined with methylprednisolone

and methylprednisolone alone

respectively

and the drug resistance was compared with the control group. The resistant strains were passaged for 30 generations in drug-free medium

and the minimum inhibitory concentration (MIC) of each passage was detected.       Results      The control group and the methylprednisolone alone group had no obvious changes in the MIC during the induction. There were 12 resistant strains (75%) in the fluconazole alone group

and 16 strains (100%) in the fluconazole combined with methylprednisolone group. After 30 passages

there were 6 resistant strains (37.5%) in the fluconazole alone group

and 9 resistant strains (56.25%) in the fluconazole combined with methylprednisolone group.       Conclusion      Methylprednisolone is helpful for increasing the in vitro induction successful rate and stability of fluconazole-resistant T. asahii. Fluconazole combined with methylprednisolone is superior to fluconazole alone in construction of fluconazole-resistant T. asahii.