Objective      To identify the species of anopheles mosquitoes from the China-Myanmar bordering area based on morphological characters and sequence analysis of the second internal transcription spacer (ITS2) of ribosomal DNA.       Methods      The anopheles mosquitoes were collected from the bordering area of China and Myanmar. Firstly

the mosquito species were identified according to the morphological characters. Then

the genomic DNA was extracted from the anopheles mosquitoes. The ITS2 sequences of the anopheles mosquitoes were obtained by PCR amplification and sequencing. And the ITS2 sequences were analyzed by online BLAST method. Finally

the ITS2 sequences were aligned with their homologs of other anopheles obtained from GenBank

and the phylogenetic tree was built using MEGA5 software.       Results      The ITS2 sequence lengths of Anopheles  minimus

Anopheles peditaeniatus and Anopheles kochi identified morphologically were 506  bp

575  bp and 517  bp

respectively. The results of BLAST showed that the molecular identification results of the anopheles species according to the ITS2 sequences were the same as the morphological identification results. The phylogenetic tree showed that the collected Anopheles  minimus

Anopheles peditaeniatus and Anopheles kochi formed the separate branches.       Conclusion      In the present study

some anopheles species in the bordering area of China and Myanmar are identified as Anopheles  minimus

Anopheles peditaeniatus and Anopheles kochi by both morphological and molecular biological methods.