Objective      To detect the biochemical parameters

insulin content in serum and tissue and mRNA level of insulin upon injection of cocaine and amphetamine regulated transcript (CART) via lateral ventricle for 24 h in normal rats and type 2 diabetes rats

and to explicit the role of CART on insulin secretion in pancreas.       Methods      Type 2 diabetes model was established by food with high fat and sugar/STZ. After i

njection of artificial cerebrospinal fluid (ACSF) or CART via lateral ventricle for 24 h

biochemical parameters and insulin content of both tissue and serum were detected. CART and insulin positioning analysis was accomplished by double immunofluorescent labeling. Insulin mRNA level in pancreas was measured by quantitative RT-PCR.       Results      Compared with ACSF injection

fasting blood glucose (FBG) decreased in type 2 diabetes rats after CART injection (

P

0.05)

although it did not return to the normal level. Furthermore

the mRNA level of insulin in pancreas increased significantly after injection of CART in type 2 diabetes rats (

P

0.05).       Conclusion      Both CART and insulin contents increase obviously in pancreas after CART injection in lateral ventricle of type 2 diabetes rats. CART may play important roles in enhancing insulin secretion in pancreas.