慢性间断性缺氧对大鼠脑缺血再灌注NF-κB及其靶基因TNF-α表达的影响

杨美丽; 黄银辉; 姚力; 曾奕明; 卓识途; 李明媚; 许盈盈; 张碧月; 蔡若蔚

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慢性间断性缺氧对大鼠脑缺血再灌注NF-κB及其靶基因TNF-α表达的影响

论著 | 更新时间：2026-01-09

- 慢性间断性缺氧对大鼠脑缺血再灌注NF-κB及其靶基因TNF-α表达的影响
- Effect of chronic intermittent hypoxia on NF-κB and TNF-α expression in cortex of rat after cerebral ischemia reperfusion injury
- 陆军军医大学学报 2014年36卷第12期页码：1303-1306
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  中图分类号： R364.4;{R392.13};R743.31
- 纸质出版：2014
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杨美丽,黄银辉,姚力,曾奕明,卓识途,李明媚,许盈盈,张碧月,蔡若蔚.慢性间断性缺氧对大鼠脑缺血再灌注NF-κB及其靶基因TNF-α表达的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(12):1303-1306.

　Yang Meili,Huang Yinhui,Yao Li,Zeng Yiming,Zuo Shitu,Li Mingmei,Xu Yingying,Zhang Biyue,Cai Ruowei.Effect of chronic intermittent hypoxia on NF-κB and TNF-α expression in cortex of rat after cerebral ischemia reperfusion injury[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(12):1303-1306.
杨美丽,黄银辉,姚力,曾奕明,卓识途,李明媚,许盈盈,张碧月,蔡若蔚.慢性间断性缺氧对大鼠脑缺血再灌注NF-κB及其靶基因TNF-α表达的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(12):1303-1306. DOI：

　Yang Meili,Huang Yinhui,Yao Li,Zeng Yiming,Zuo Shitu,Li Mingmei,Xu Yingying,Zhang Biyue,Cai Ruowei.Effect of chronic intermittent hypoxia on NF-κB and TNF-α expression in cortex of rat after cerebral ischemia reperfusion injury[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(12):1303-1306. DOI：

摘要

目的观察大鼠脑皮质炎症因子[核转录因子κB (NF-κB p65)

]

及其靶基因TNF-α的表达，探讨慢性间断缺氧对脑缺血再灌注后炎症反应影响。方法将SD大鼠24只按随机数字表法分为空白对照组、慢性间断缺氧组、线栓组、慢性间断缺氧+线栓组，每组6只。建立SD大鼠慢性间断缺氧联合局灶性脑缺血再灌注模型，免疫组化染色检测脑皮质中NF-κB p65、TNF-α的表达。结果与空白对照组比较，CIH、CIR、CIH+CIR均可促使NF-κB p65及TNF-α的表达增多，差异存在统计学意义（

0.05）；CIH与CIR存在交互作用（

0.05），CIH+CIR与CIH及CIR比较可促使NF-κB p65及TNF-α表达明显增多，差异存在统计学意义（

0.05）。大鼠脑皮质NF-κB p65与TNF-α呈正相关(r=0.90

0.01)。结论慢性间断性缺氧可使大鼠脑缺血再灌注NF-κB p65及其靶基因TNF-α的表达显著增多。

Abstract

Objective To study the roles of chronic intermittent hypoxia (CIH) in the expression of inflammatory cytokines

nuclear factor-κB (NF-κB) p56 and tumor necrosis factor-α (TNF-α)

in the brain cortices of rats with cerebral ischemia reperfusion (CIR) injury. Methods In this experiment

36 Sprague-Dewley rats were randomly divided into 4 groups

a control group

a CIH group

a CIR group

and a CIH+CIR group. The rat model of CIH was made

and then the rats were exposed to CIR injury. The effusion of polymorphonuclear leucocytes in the brain tissue

was detected by HE staining. The expression of NF-κB p65 and TNF-α in the brain cortex was detected by immunohistochemistry assay. Results The expression of NF-κB p65 and TNF-α in the cortices of rats was significantly higher in the CIH group

the CIR group and the CIH+CIR group than in the control group (

0.05). Moreover

there was an interactive activity between CIH and CIR (

0.05). The expression of NF-κB p65 and TNF-α in the cortices of rats was significantly higher in the CIH+CIR group than in the CIH group and the CIR group (

0.05). The expression of NF-κB p65 was positively correlated with the expression of TNF-α (r=0.90

0.01). Conclusion CIH increases the expression of NF-κB p65 and TNF-α in the cortices of rats with CIR injury.

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