Objective       To identify the core promoter region of lung cancer drug resistance-related gene CA916798 preliminarily.       Methods       The upstream sequence 0H to 9H containing different fragments of CA916798 were amplified by PCR

and then the products were cloned into pGL3-basic plasmid. Then the obtained plasmids were transfected into COS7 cells. Promoter activities were evaluated by luciferase test. The possible position of core promoter was identified by endonuclease digestion

sequencing and BLAST. The binding sites of core promoter region were predicted by bioinformatics.       Results       Target fragments were successfully cloned.

The relative fluorescence intensity of group 9H was significantly higher than negative group (

P

0.05). As compared with group 0H to 8H

the relative fluorescence intensity were also markedly increased in group 9H (

P

0.05). 9H fragment was 568 bp

which located at 179 bp upstream of CA916798. The binding sites for several transcriptional factors were detected in the process of function prediction.       Conclusion       9H fragment has a strong promoter activity

and the sequence is the major transcriptional locus of CA916798.