Objective        To determine the effect of the over-expression of dual specificity phosphatase 5 (DUSP5) on the proliferation

apoptosis in human placental trophoblast cell line BeWo and investigate the possible signaling pathway.       Methods        BeWo cells was infected by recombinant lentiviral vector carrying human DUSP5 gene. The cells infected by empty lentiviral vector and untreated cells served as co

ntrol. Flow cytometry was used to select the cells over-expressing DUSP5. Real-time PCR and Western blotting were used to detect the mRNA and protein levels of of DUSP5. CCK-8 assay and flow cytometry were employed to detect cell proliferation and apoptosis. The expression of p-ERK1/2

p-p38 and p-JNK1/2/3 were detected by Western blotting.       Results        Real-time PCR and Western blotting indicated that the BeWo cells over-expressing DUSP5 was successfully established. CCK-8 assay confirmed that the BeWo cells over-expressing DUSP5 had significantly higher rates of proliferation than the other 2 cells in days 3

4 and 5 (

P

0.01). The number of normal cells was significantly increased

while that of early apoptotic cells was markedly lesser in DUSP5 over-expression cells than the vector group in 48 h after cisplatin treatment (

P

0.01). The protein level of p-ERK1/2 was lower in BeWo cells over-expressing DUSP5 than in the cells infected by empty lentiviral vector and untreated cells

while that of p-p38 and p-JNK was not changed.       Conclusion        Over-expressing of DUSP5 induces cell proliferation

and inhibits cell apoptosis in human placental trophoblast BeWo cells

which may be relevant with inhibiting ERK1/2 signaling pathway.