FGF2对破骨细胞体外分化以及骨吸收功能的直接调控作用

李晓刚; 苏楠; 唐玉彬; 温轩; 唐浚洲; 陈思宇; 李灿; 杜晓兰; 陈林

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FGF2对破骨细胞体外分化以及骨吸收功能的直接调控作用

论著 | 更新时间：2026-01-09

- FGF2对破骨细胞体外分化以及骨吸收功能的直接调控作用
- Fibroblast growth factor 2 directly regulates osteoclast differentiation and bone resorption function
- 陆军军医大学学报 2014年36卷第7期页码：636-639
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  中图分类号： R322.71;R329.25;R341
- 纸质出版：2014
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李晓刚,苏楠,唐玉彬,温轩,唐浚洲,陈思宇,李灿,杜晓兰,陈林.FGF2对破骨细胞体外分化以及骨吸收功能的直接调控作用[J].陆军军医大学学报(原第三军医大学学报),2014,36(07):636-639.

　Li Xiaogang,Su Nan,Tang Yubin,Wen Xuan,Tang Junzhou,Chen Siyu,Li Can,Du Xiaolan,Chen Lin.Fibroblast growth factor 2 directly regulates osteoclast differentiation and bone resorption function[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(07):636-639.
李晓刚,苏楠,唐玉彬,温轩,唐浚洲,陈思宇,李灿,杜晓兰,陈林.FGF2对破骨细胞体外分化以及骨吸收功能的直接调控作用[J].陆军军医大学学报(原第三军医大学学报),2014,36(07):636-639. DOI：

　Li Xiaogang,Su Nan,Tang Yubin,Wen Xuan,Tang Junzhou,Chen Siyu,Li Can,Du Xiaolan,Chen Lin.Fibroblast growth factor 2 directly regulates osteoclast differentiation and bone resorption function[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(07):636-639. DOI：

摘要

目的研究成纤维细胞生长因子2（fibroblast growth factor 2，FGF2）对于破骨细胞（osteoclast，OC）体外分化与功能的直接调控作用。方法分离野生型C3H/HeJ小鼠骨髓单核细胞，利用核因子κB受体活化因子配体（receptor activator of NF-κB ligand，RANKL）以及巨噬细胞集落刺激因子（macrophage colony stimulating factor，M-CSF）诱导向破骨细胞分化，同时在实验组中加入FGF2，观察骨髓单核细胞向破骨细胞分化发育过程，鬼笔环肽（phalloidine）染色观察破骨细胞肌动蛋白环（actin ring）变化，Real-time PCR检测破骨细胞Trap、Mmp9、Ctsk以及Clcn7表达，破骨细胞与骨片共培养检测骨吸收功能变化，并用Western blot检测相关信号通路分子表达情况。结果 ①TRAP染色显示实验组与对照组破骨细胞数量无明显差异（

0.05）；②鬼笔环肽染色提示实验组与对照组肌动蛋白环均正常；③实验组Trap、Mmp9及Ctsk mRNA表达量均高于对照组（

0.05），而Clcn7 mRNA表达2组无明显差异；④体外骨片吸收实验提示实验组骨吸收陷窝面积显著大于对照组（

0.05）；⑤Western blot检测实验组磷酸化细胞外信号调节激酶（ERK）较对照组表达明显增多。结论 FGF2不影响破骨细胞前体细胞向破骨细胞的分化以及肌动蛋白环的形成，但可以通过ERK信号通路正向调控成熟破骨细胞的骨吸收功能。

Abstract

Objective To determine the effect of fibroblast growth factor 2 (FGF2) on the differentiation of bone marrow monocytes (BMMCs) to osteoclast and bone resorption function. Methods After BMMCs were isolated from the bone marrow of femurs and tibiae from C3H mice

the cells were treated with receptor activator of nuclear factor-kappa B ligan

d (RANKL) and macrophage colony stimulating factor (M-CSF). Experimental and control groups were separated by addition of FGF2 or not. The differentiation and maturation of osteoclast precursors were observed by TRAP staining

and the osteoclast actin ring of both group were analyzed by phallodine staining. The expression of Trap

Mmp9

Ctsk and Clcn7 at mRNA level were detected by real-time PCR. The function of bone resorption was measured by bovine bone slice and osteoclast coculture. The effect of FGF2 on activity of ERK protein in the osteoclasts was analyzed by Western blotting. Results There was no significant difference in differentiation and maturation of osteoclast precursors between 2 groups (

0.05)

neither in the actin ring of osteoclasts. The mRNA expression level of Trap

Mmp9

and Ctsk were upregulated after FGF2 treatment (

0.05)

however

Clcn7 mRNA expression level was not influenced. Compared with control group

the bone resorption lacuna area of FGF2 treated group was larger (

0.05). Finally

Western blot analysis showed that treatment with FGF2 induced enhanced activity of phosphorylated ERK in cultured osteoclasts. Conclusion FGF2 cannot influence the differentiation of osteoclast precursors or formation of actin ring

but can upregulate the function of osteoclastic resorption via an ERK-related pathway.

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