Objective      To investigate the effects of advanced glycation end-products(AGEs) on the expression of synaptopodin and podocyte migration

and to explore their mechanisms.       Methods      Podocytes were incubated with different concentrations of AGEs （0，20，40 and 80 μg/mL）for 24 h

the expression levels of synaptopodin were measured by Western blotting

and cytoskeleton reorganization were observed by laser scanning confocal microscopy. Podocyte migration w

ere assayed by Transwell assay.Then podocytes were pretreated with losartan for 60 min

and the changes of synaptopodin and cytoskeleton were observed.       Results      Incubation with AGEs resulted in a significant decrease in the expression of synaptopodin[（50±11）% vs 100%

P

0.05

]

AGEs also induced F-actin reorganization and increased podocyte migration(47±6 vs 13±3

P

0.05). However

pretreatment with losartan (100 μmol/L)alleviated the effects induced by AGEs in podocytes.       Conclusions      AGEs downregulate the expression of  synaptopodin

induce cytoskeleton reorganization

and increase podocyte migration via activation the local renin-angiotensin system in podocytes.