Objective      To investigate the effects of T cell factor 4 (TCF4) siRNA on TCF4 gene expression

and the migration

invasion and cell cycle of human squamous carcinoma A431 cells.       Methods      A431 cells were transfected with TCF4 siRNA liposome. The mRNA expression of TCF4 in A431 cells was detected by RT-PCR. The expression of TCF4 protein was detected by Western blotting. The proliferation

migration

and invasion activities and cell cycle of A431 cells were measured by CCK-8 assay

chamber cell migration assay

transwell chamber test and flow cytometry

respectively.     

  Results      TCF4 siRNA transfection could inhibit significantly the expression of TCF4 mRNA and protein in A431 cells. The rates of cell proliferation and abilities of migration and invasion were also significantly decreased after TCF4 siRNA transfection. In addition

analysis of cell cycle demonstrated a marked G

2

/M arrest of the A431 cells treated with TCF4 siRNA.       Conclusion      It is concluded that TCF4 siRNA can inhibit proliferation of human squamous carcinoma cells

which may provide a rational approach in clinical therapy for human squamous carcinoma.