Objective       To establish Hedgehog signaling pathway detection method for Shh gene and Smo gene using real-time PCR quantitatively

and analyze their expression levels in chronic myeloid leukemia (CML) patients.       Methods       Using Real-time PCR with SYBR Green Ⅰ technique

Shh gene and Smo gene expression levels in peripheral blood mononuclear cells from 8 normal individuals (control) and 20 CML patients was detected with β2-microglobulin (β2M) as reference to acquire the relative expression levels.       Results       Shh and Smo expression levels in CML patients were significantly higher than those in the normal control (

P

0.05).       Conclusion       The quantitative detection method for Shh and Smo gene expression by real-time PCR with SYBR Green Ⅰ

technique has been successfully established. Shh gene and Smo gene are highly expressed in CML patients.