牛蒡子苷自微乳在体肠吸收分析方法学研究

赵德华; 卢来春; 管海燕; 张卿; 尚京川

您当前的位置：

首页 >

文章列表页 >

牛蒡子苷自微乳在体肠吸收分析方法学研究

论著 | 更新时间：2026-01-09

- 牛蒡子苷自微乳在体肠吸收分析方法学研究
- Analytical methodology on in situ rat intestine absorption of arctiin SMEDDS
- 陆军军医大学学报 2013年35卷第24期页码：2681-2685
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R282.71;R944.9;R965
- 纸质出版：2013
- 稿件说明：
移动端阅览
赵德华,卢来春,管海燕,张卿,尚京川.牛蒡子苷自微乳在体肠吸收分析方法学研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(24 ):2681-2685.　Zhao Dehua,Lu Laichun,Guan Haiyan,Zhang Qing,Shang Jingchuan.Analytical methodology on in situ rat intestine absorption of arctiin SMEDDS[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(24 ):2681-2685.

赵德华, 卢来春, 管海燕, et al. Analytical methodology on in situ rat intestine absorption of arctiin SMEDDS[J]. Journal of Army Medical University, 2013, 35(24): 2681-2685.
赵德华,卢来春,管海燕,张卿,尚京川.牛蒡子苷自微乳在体肠吸收分析方法学研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(24 ):2681-2685.　Zhao Dehua,Lu Laichun,Guan Haiyan,Zhang Qing,Shang Jingchuan.Analytical methodology on in situ rat intestine absorption of arctiin SMEDDS[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(24 ):2681-2685. DOI：

赵德华, 卢来春, 管海燕, et al. Analytical methodology on in situ rat intestine absorption of arctiin SMEDDS[J]. Journal of Army Medical University, 2013, 35(24): 2681-2685. DOI：

摘要

目的建立HPLC法测定牛蒡子苷自微乳中牛蒡子苷含量并考察牛蒡子苷自微乳在大鼠小肠吸收情况。方法采用自微乳化释药系统（SMEDDS）技术制备牛蒡子苷自微乳，并运用大鼠在体灌流的方法建立牛蒡子苷大鼠肠吸收模型，以流动相乙腈-水（35∶65），流速1 mL/min，检测波长280 nm为色谱条件，测定自微乳中牛蒡子苷含量及肠循环液中牛蒡子苷、苷元含量，并考察药物的吸收。结果牛蒡子苷含量测定方法在2～200 μg/mL浓度范围内，苷元含量测定方法在1～100 μg/mL浓度范围内，均具有良好线性，回收率均在95%～105%范围内，日内及日间RSD均小于5%。测得牛蒡子苷自微乳载药量为（40.090±0.001）mg/g；在体肠吸收结果表明在10～50 μg/mL范围内，药物单位时间吸收百分率小于25%；牛蒡子苷自微乳在小肠有一定的吸收，但在30 min后开始水解为牛蒡子苷元。结论所建HPLC法专属性强，准确度及精密度能够满足牛蒡子苷自微乳以及在体肠吸收液中牛蒡子苷、苷元含量的测定。

Abstract

Objective To establish an HPLC method for determination of arctiin in self-microemulsifying drug delivery system (SMEDDS) and studying its absorption in rat intestines. Methods An in situ recirculation model was established to study the absorption of arctiin SMEDDS in rat intestines. The contents of arctiin and arctigenin and drug absorption were determined by HPLC

with mobile phase of acetonitrile-water (35∶65)

flow rate 1 mL/min

and detection wavelength 248 nm. Results The method showed good linearity in arctiin concentration range of 2-200 μg/mL and arctigenin concentration range of 1-100 μg/mL

with recovery rate 95% to 105% and inter-day and intra-day RSD less than 5%. The content of arctiin in microemulsion was (40.090±0.001) mg/g. In the concentration range from 10 to 50 μg/mL

arctiin absorption rate was less than 25%. The arctiin SMEDDS had some absorption in rat intestines but started to hydrolyze and produce arctigenin after 30 min. Conclusion The HPLC method with good specificity

accuracy and precision is qualified for determination of arctiin and arctigenin from SMEDDS and intestinal fluids.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

HPLC法测定盐酸贝那替秦原料药及其注射剂的含量和有关物质

辐照对去甲肾上腺素注射液含量变化的影响

氟比洛芬口腔崩解片的制备及质量控制

紫杉醇提取工艺的优化试验研究