Objective       To determine the effect of artificially synthesized sodium channel amine ligands (SCALs) on the growth and invasion of prostate cancer cell lines.       Methods       SCALs compounds

S1127

S1156

S1169

S1170

S1178

S1180

and S1182 were synthesized and confirmed. Prostate cancer cell lines

PC3 and DU145

were used in this study. The cell viability was assessed by MTT assay in the cells after the treatment of SCALs respectively. The cell apoptosis and cell cycle progression were assessed by flow cytometry. T

he cancer cell invasion was assessed by Transwell chamber assay. The protein expression of prostate cancer sodium channel α subunit

Nav 1.7 was determined by Western blotting.       Results       Three compounds

S1127

S1169

and S1180

inhibited the growth of tumor cells in a dose-dependent manner. S1180 was found to be the most potent among the 7 compounds. The IC

50

values of S1180 were 2.9 and 2.5 μmol/L respectively for PC3 and DU145 cells. At 10 μmol/L

S1180 improved cell apoptosis in 12.89% PC3 cells

and suppressed the cell invasion in 75.53% cells

and exerted significant effects on cell arrest

and down-regulated Nav 1.7 protein expression.       Conclusion       Three SCALs compounds

especially S1180

have obvious anti-tumor effect on prostate cancer cells

and might be a potential therapeutic agent for prostate cancer.