Objective      To determine the effect of gasdermin A3 (Gsdma3) gene on NF-κB expression in mouse skin keratinocytes.       Methods      Histological section and immunofluorescent assays were performed to locate and measure NF-κB expression between the Gsdma3-mutant mouse skin and the normal one. NF-κB expression was revealed by immunofluorescent assay after Gsdma3 expression plasmid was transfected into mouse skin keratinocytes. RT-PCR and Western blotting were used in vivo and in vitro to investigate NF-κB expr

ession at mRNA  and protein levels

respectively

in Gsdma3-mutant or Gsdma3-overexpressed skin keratinocytes.       Results      In mouse skin keratinocytes

Gsdma3 mutation decreased NF-κB  mRNA expression by 39.53% (

P

0.01) and  protein expression by 65.03% (

P

0.01). Positive zones and intensity of immunofluorescence were significantly mitigated in Gsdma3-mutant skin keratinocytes as compared to the normal ones. On the other hand

overexpression of Gsdma3 could increase the  mRNA expression of NF-κB by 56.25% (

P

0.01) and the  protein expression by 199.6% (

P

0.01). In addition

Gsdma3 and NF-κB exhibited an opposite expression manner in the in vitro immunofluorescent assays.       Conclusion      Gsdma3 can induce mRNA and protein expression of NF-κB in the mouse skin keratinocytes.