Objective      To study the function of BCR-ABL protein C-terminal actin-binding domain (ABD) on cell cycle and apoptosis in K562 cells and HL60 cells

and to provide the experimental basis for chronic myeloid leukemia (CML) treatment.       Methods      The recombinant expression vector harboring ABD was constructed

and then recombinant proteins were transferred into leukemia cells with protein transduction domain (PTD). The localization of recombinant proteins inside the leukemia cells was confirmed by Western

blotting and immunofluorescence assay. Growth curves and apoptosis of leukemia cells transfected by PTD-ABD fusion protein were determined by MTT assay and flow cytometry respectively.        Results      The PTD-ABD and PTD recombinant expression vectors were constructed successfully

and proteins with high purity were obtained. The growth of HL60 cells was inhibited and the apoptosis was accelerated by PTD-ABD fusion protein. The difference between experimental and control groups had statistical significance (

P

0.05). However

PTD-ABD fusion protein showed no difference on K562 cells (

P

0.05). In addition

PTD protein had no effect on the apoptosis and growth of K562 and HL60 cells in comparison with negative control (

P

0.05).       Conclusion      PTD-ABD fusion protein inhibits the growth and induces the apoptosis  in HL60 cells.