Objective       To observe the expression of Rac1 in human ovarian serous adenocarcinoma tissues and its role in the malignant proliferation and migration of ovarian cancer cell SKOV3.       Methods        Western blotting was applied to detect the expression of Rac1 protein in ovarian serous adenocarcinoma tissues

benign ovarian serous cystadenoma tissues and normal ovarian tissues. Stable Rac1-silencing SKOV3 cells were constructed and named as SKOV3-Rac1i. Wound healing assay was conducted to evaluate the ability of the cell migration

and MTT assay and plate

clone assay were performed to detect the proliferation rate of SKOV3-Rac1i cells.       Results      The expression level of Rac1 protein was significantly higher in human ovarian serous adenocarcinoma tissues than in benign ovarian serous cystadenoma tissues (0.89±0.17 vs 0.69±0.24

P

0.05) and normal ovarian tissues (0.89±0.17 vs 0.72±0.12

P

0.05). Moreover

suppressed migration and proliferation were observed in SKOV3-Rac1i cells (

P

0.05).       Conclusion      Rac1 is highly expressed in human ovarian serous adenocarcinoma tissues

and suppressed migration and proliferation of SKOV3-Rac1i cells support its contribution to malignant biological behavior of ovarian cancer cells.