Objective        To study the effect of potassium channel tetramerisation domain containing 15 (KCTD15) gene on preadipocyte differentiation.       Methods        The expression of KCTD15 gene during 3T3-L1 preadipocyte differentiation was detected by semi-quantitative reverse transcriptase PCR. After transferring KCTD15 siRNA into the preadipocytes

the cell morphology was observed during preadipocyte differentiation by oil red O staining

and the level of triglyceride was examined by assay kit. The expression of adipogenesis genes

peroxisome proliferator-activated receptor (PPAR)γ

CCAAT/enhancer-binding protein (C/EBP)α

C/EBPβ and C/EBPδ was detected by semi-quantitative reverse transcriptase PCR.       Results        The expression of KCTD15 gene was decreased during 3T3-L1 cell differentiation. KCTD15 gene knockdown inhibited the differentiation and lipid accumulation of 3T3-L1 cells

and there was no significant change in the expression of PPARγ

C/EBPα

C/EBPβ and C/EBPδ.       Conclusion        KCTD15 gene deficiency leads to the inhibition of 3T3-L1 preadipocyte differentiation at early stage.