Objective      To develop a quantitative retention-activity relationship (QRAR) model by biopartitioning micellar chromatography (BMC) for estimating the pharmacokinetic parameters of flavonoids.       Methods      The retention time of the flavonoids in Brij 35 buffer solution (0.05 mol/L

pH 7.4) were determined

and the retention data of flavonoids and their corresponding properties were adjusted to a second-order polynomial QRAR model

of which the interpretative and predictive abilities were evaluated.     

  Results      The QRAR models of half-life period (T

1/2

)

apparent volume of distribution (Vd) and systemic clearance (Cl) obtained with the optimized chromatography conditions were statistically significant in the correlation coefficients

which were 0.938

0.898 and 0.837 with the standard deviation of 41.929

19.528 and 0.139

respectively. The comparability of cross validation results met the statistical requirement for good prediction.       Conclusion      The pharmacokinetic QRAR model obtained using the retention data of flavonoids shows good interpretative and predictive abilities.