Objective      To explore the efficiency of siRNA delivery by using nanopatch and the inhibitory effect of HPV18 E7 siRNA (siE7) on the proliferation of skin cells in cervical cancer transgenic mice.       Methods      The oligonucleotide tagged with FITC as a substitute of siRNA was delivered into mouse skin by nanopatch

and the green fluorescence was observed to analyze whether the nanopatch can deliver siRNA into the cells of mouse skin. Nanopatch was used to deliver the complexes of siE7 and liposomes marked with DiI into mouse skin. The fluorescence intensity of DiI before and after transfection and that remained on the surface of mouse skin after transfection were determined. The efficiency of siE7 delivery by nanopatch was calculated

and BrdU immunohistochemistry was applied to detect the proliferation of skin cells transfected with siE7 in cervical cancer transgenic mice.       Results      The green fluorescence detected by confocal laser scanning microscopy (CLSM) after transfection by nanopatch increased and scattered

suggesting the oligonucleotide was delivered into skin cells. The transfection efficiency of using nanopatch to deliver siRNA was 50.23％. The proliferation of transgenic mouse skin cells transfected with siE7 by nanopatch was inhibited.       Conclusion      Nanopatch can effectively deliver siRNA into mouse skin cells

and siE7 delivered by nanopatch can inhibit the proliferation of skin cells of cervical cancer transgenic mice.