Objective        To investigate the effects of S100A4 on the proliferation

migration

invasion and apoptosis of HBL-100 and MCF-7 cell lines

and t

o explore the effects of S100A4 on Wnt/β-catenin signaling pathway and Wnt/JNK signaling pathway in the two cell lines.       Methods      The recombinant retrovirus that carries human S100A4 gene (AdS100A4) was used to infect HBL-100 and MCF-7 cells

and the retrovirus carrying GFP (AdGFP) was used as control. The effects of S100A4 on the proliferation

migration

invasion and apoptosis of HBL-100 and MCF-7 cells were detected by MTT assay

wound healing assay

Transwell invasion assay and Hoechst staining

respectively. The effects of S100A4 on the activities of Wnt/β-catenin signaling pathway and Wnt/JNK signaling pathway were detected by PCR and Western blotting.       Results      (1) The activities of cell proliferation  were increased by 53.3% and 59.1% at 4 d after infection for HBL-100 and MCF-7 cells

respectively (

P

0.05)

and the wound healing rates were increased by 73.6% and 64.8%

respectively  at 24 h after infection (

P

0.05). Compared with the AdGFP group

the number of transmembrane HBL-100 cells had no significant difference (

P

0.05)

but the number of transmembrane MCF-7 cells was doubled (

P

0.05). The apoptotic rates of HBL-100 and MCF-7 cells at 48 h after infection were decreased by about 30% and 36%

respectively (

P

0.05). (2) Compared with the AdGFP group

the β-catenin level in HBL-100 and MCF-7 cells was increased by 49% and 55%

respectively (

P

0.05)

the c-Myc level increased by 38% and 30%

respectively (

P

0.05)

and the p-GSK3β level were increased by 73% and 55%

respectively (

P

0.05)

with no significant change of t-GSK3β level. (3) The mRNA levels of t-JNK

p-JNK and c-Fos in HBL-100 cells as well as the mRNA level of t-JNK in MCF-7 cells did not change after infection (

P

0.05)

but the mRNA levels of p-JNK and c-Fos were  increased by 39% and 32.3% in HBL-100 and MCF-7 cells

respectively (

P

0.05).       Conclusion      (1) S100A4 can promote cell proliferation and migration

and inhibit apoptosis in HBL-100 and MCF-7 c

ell lines. (2) S100A4 can promote invasion of MCF-7 cell lines. (3) S100A4 can enhance the activities of Wnt/β-catenin signaling pathway in HBL-100 and MCF-7 cells as wells as the activity of Wnt/JNK signaling pathway in MCF-7 cells.