HBx及其突变体X17-3对Hippo信号途径表达的影响

陈琰琰; 朱慧芬; 杨道锋; 刘晓聪; 邓科兰; 黄春梅; 胡晓萌; 姜素华; 沈关心

您当前的位置：

首页 >

文章列表页 >

HBx及其突变体X17-3对Hippo信号途径表达的影响

论著 | 更新时间：2026-01-09

- HBx及其突变体X17-3对Hippo信号途径表达的影响
- HBx and its mutant X17-3 regulates YAP through Hippo signaling pathway in human hepatic L02 cells
- 陆军军医大学学报 2013年35卷第16期页码：1704-1707
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R322.47;R329.26;R394.2
- 纸质出版：2013
- 稿件说明：
移动端阅览
陈琰琰,朱慧芬,杨道锋,刘晓聪,邓科兰,黄春梅,胡晓萌,姜素华,沈关心.HBx及其突变体X17-3对Hippo信号途径表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(16):1704-1707.

　Chen Yanyan,Zhu Huifen,Yang Daofeng,Liu Xiaocong,Deng Kelan,Huang Chunmei,Hu Xiaomeng,Jiang Suhua,Shen Guanxin.HBx and its mutant X17-3 regulates YAP through Hippo signaling pathway in human hepatic L02 cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(16):1704-1707.
陈琰琰,朱慧芬,杨道锋,刘晓聪,邓科兰,黄春梅,胡晓萌,姜素华,沈关心.HBx及其突变体X17-3对Hippo信号途径表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(16):1704-1707. DOI：

　Chen Yanyan,Zhu Huifen,Yang Daofeng,Liu Xiaocong,Deng Kelan,Huang Chunmei,Hu Xiaomeng,Jiang Suhua,Shen Guanxin.HBx and its mutant X17-3 regulates YAP through Hippo signaling pathway in human hepatic L02 cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(16):1704-1707. DOI：

摘要

目的初步探寻HBx及其不同突变体表达与Hippo信号途径的关系，以及对细胞凋亡的影响。方法 HBx及其突变体载体转染人正常肝细胞系L02，转染48 h后，提取总蛋白，Western blot检测细胞中Hippo信号途径MST1、YAP(yes-associated protein)的表达，磷酸化和去磷酸化的情况。采用Annexin V/PI标记流式细胞术检测细胞凋亡。结果 HBx及其突变体载体转染L02细胞48 h后，Western blot结果显示细胞MST1表达下调，p-MST1/2表达上升（

0.05）；YAP表达上升，p-YAP表达下调（

0.05）。Annexin V/PI标记法流式细胞术结果显示HBx及其突变体能促进L02细胞发生凋亡（

0.05），其介导的细胞早期凋亡尤为明显。结论 HBx通过Hippo信号通路途径调控下游致癌基因YAP的表达。结合HBx介导的L02细胞凋亡这一结果说明HBx可能通过多种途径调节细胞周期。

Abstract

Objective To investigate the relationship of HBx and its mutant X17-3 with Hippo signaling pathway

and the effects of HBx on cell cycle and apoptosis. Methods L02 cells were transfected with HBx and its mutant

respectively. After 48 h of incubation

the expression levels as well as the phosphorylation status of MST1/2 and yes-associated protein (YAP) were detected by Western blotting. The apoptosis of L02 cells was examined with flow cytometry by Annexin V/PI staining. Results After 48 hours’ transfection

Western blotting showed that the expression levels of p-MST1/2 and

YAP were up-regulated

and those of MST1/2 and p-YAP were down-regulated (

0.05). The results of flow cytometry indicated that the transfection of HBx and X17-3 resulted in more apoptotic cells （

0.05）

especially in early stage of apoptosis. Conclusion HBx regulates its downstream oncogene

YAP through Hippo signaling pathway. Its also regulates the cell cycle via several ways.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

肉桂醛对糖尿病溃疡血管内皮细胞Bax/Bak和凋亡的影响

SRSF1通过增加P53的稳定性抑制乙型肝炎病毒复制

ETS1转录上调FBXO45通过EMT而促进肝癌的侵袭迁移

过度机械拉伸应力通过Piezo1介导肌腱细胞凋亡的作用及机制研究

高龄孕鼠子代脑海马星形胶质细胞和神经元初级纤毛功能缺陷可能与LKB1/AMPK通路促进凋亡相关