Objective      To prepare a three-dimensional chitosan scaffold loading with transforming growth factor-β3 (TGF-β3) microspheres.       Methods      Three-dimensional chitosan scaffold was prepared by lyophilization

and water bonding capacity and porosity were measured. TGF-β3 loaded chitosan microspheres were prepared by emulsion-crosslinking method. The controlled release of TGF-β3 was monitored for 7 d and the swelling index was measured. At last

the three-dimensional chitosan scaffold loading with TGF-β3 microspheres was prepared and observed by scanning electron microscope (SEM).        Results      SEM images showed the morphology of porous scaffold was uniform

and the mean diameter of interconnected pores was 180.4±35.3 μm. The porosity was (83.2±0.6)% and swelling index of microspheres was (123±5)%. The microspheres were spherical and smooth

and the mean diameter of the microspheres was 28.5±5.1 μm. The accumulated release of TGF-β3 reached (46.2±0.3)% within 7 d. Most microspheres were uniform in the scaffold.       Conclusion      The method for preparing three-dimensional chitosan scaffold loading with TGF-β3 microsphere is feasible

and the sustained release of TGF-β3 is realized.