Objective      To investigate the protective effect of different doses of tea polyphenol (TP) on the mouse sertoli cells against oxidative damage and apoptosis induced by glyphosate (GLY).       Methods      The oxidative damage model of primarily culture

d mouse sertoli cells was established through GLY (90 mg/L) incubation for 24 h. Then the cells were treated with different doses of TP (10

20

40 and 80 mg/L). Meanwhile

the control cells were incubated with normal medium. After incubation for 24 h

the sertoli cells were observed for the morphological changes by inverted microscopy. Cell viability was evaluated by MTT assay. Cell cytotoxicity was measured by lactate dehydrogenase (LDH) test. Cell apoptosis was tested with TUNEL assay. The activity of super oxidase dismutase (SOD)

as well as the levels of glutathione (GSH）and malondialdehyde (MDA）were also detected.       Results       After the sertoli cells were treated with GLY (90 mg/L)

the cells became smaller

and more cast-off cells and cell debris were found. The cell viability was significantly lower than that in the normal control (

P

0.05)

only accounting for 47.03%. The LDH activity and the level of MDA were significantly higher (

P

0.05)

and the activity of SOD and the level of GSH were obviously lower than those of control group. Furthermore

the rate of cell apoptosis was （36.96±4.0）%

significantly higher than that of control group (

P

0.05). Meanwhile

when sertoli cells were treated with GLY followed by different doses of TP

the states of cells were improved

especially in those treated by 80 mg/L TP

and there were less cell debris found. TP inhibited the decrease of cell proliferation

SOD activity and GSH level (

P

0.05

P

0.01). In addition

TP also inhibited the increase of MDA level and LDH activity (

P

0.05

P

0.01).       Conclusion      GLY induces apoptosis

inhibits cell proliferation

and decreases anti-oxidative ability in mouse sertoli cells. While

TP plays a protective role against these damages.