Objective      To compare the changes of extracellular matrix in decellularized articular cartilage treated by Triton X-100 and sodium dodecyl sulfate（SDS） to optimize the suitable preparation.       Methods      First

cartilage particles were prepared

then the two reagents was used to treat the cells of cartilage particles respectively

with the same experimental procedures of rinsing and digestion. After decellularization

the contents of DNA

amino acid

collagen and glycosaminoglycan  (GAG) were measured

and the results were compared with those of the normal cartilaginous tissue.       Results      After decellularization

the GAG contents were 84.71±3.72 and 122.63±6.05 μg/mg respectively in the extracellular matrix of articular cartilages treated by Triton X-100 and SDS

while that was 232.78±75.33 μg/mg in the extracellular matrix of normal cartilaginous cells. Hydroxyproline content was 24.92±1.74 and 24.23±1.68 μg/mg respectively after the decellularization

and that in the normal matrix was 24.54±2.99 μg/mg. DNA content was 0.242±0.065 and 0.225±0.057 μg/mg respectively

and that in the normal one was 5.679±1.873 μg/mg.       Conclusion      Cell components are removed effectively from cartilage particles by both Triton X-100 and SDS without obvious difference of collagen remains. While SDS reserves more GAG components and is more helpful to maintain the cartilage cells’ function. So SDS decellularization is a better preparative technique for tissue engineering.