共培养体系中卵巢癌细胞对内皮祖细胞成管及MMP-2和MMP-9表达的影响

陈振波; 赵俪梅; 罗艳梅; 阎萍; 梁志清

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共培养体系中卵巢癌细胞对内皮祖细胞成管及MMP-2和MMP-9表达的影响

论著 | 更新时间：2026-01-09

- 共培养体系中卵巢癌细胞对内皮祖细胞成管及MMP-2和MMP-9表达的影响
- Effect of ovarian cancer cells on MMP-2 and MMP-9 expression and tube formation of endothelial progenitor cells in co-culture system
- 陆军军医大学学报 2013年35卷第10期页码：956-960
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  中图分类号： R394.2;R730.23;R737.31
- 纸质出版：2013
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陈振波,赵俪梅,罗艳梅,阎萍,梁志清.共培养体系中卵巢癌细胞对内皮祖细胞成管及MMP-2和MMP-9表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(10):956-960.

　Chen Zhenbo,Zhao Limei,Luo Yanmei,Yan Ping,Liang Zhiqing.Effect of ovarian cancer cells on MMP-2 and MMP-9 expression and tube formation of endothelial progenitor cells in co-culture system[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(10):956-960.
陈振波,赵俪梅,罗艳梅,阎萍,梁志清.共培养体系中卵巢癌细胞对内皮祖细胞成管及MMP-2和MMP-9表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(10):956-960. DOI：

　Chen Zhenbo,Zhao Limei,Luo Yanmei,Yan Ping,Liang Zhiqing.Effect of ovarian cancer cells on MMP-2 and MMP-9 expression and tube formation of endothelial progenitor cells in co-culture system[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(10):956-960. DOI：

摘要

目的观察血管内皮生长因子（Vascular endothelial growth factor

VEGF）对共培养体系中（EPCs+SKOV3）内皮祖细胞（endothelial progenitor cells

EPCs）的成管能力和MMP-2、MMP-9的表达影响。方法采用密度梯度离心法分离EPCs，先在Matrigel基质胶中测定其成管能力，再与SKOV3共培养后测定成管能力，最后在共培养体系中加入不同浓度的血管内皮生长因子（Vascular endothelial growth factor

VEGF）抗体再分别测定不同浓度抗体组的EPCs成管能力。采用RT-qPCR及Western blot测定不同浓度抗体组EPCs中MMP-9、MMP-2的表达量。结果 EPCs单独成管时管腔个数为（18.00±1.00），较共培养组（36.33±1.52）明显降低（

0.05）。加入25、50、100 μg VEGF抗体的共培养组EPCs成管个数分别为 32.00±1.00、26.33±0.57、20.33±1.15，均较共培养组降低（

0.05）。RT-qPCR及Western blot结果显示：共培养组MMP-2和MMP-9 mRNA相对表达倍数较EPCs组分别上调了4.36、3.34倍(

0.05），蛋白表达量上调了2.99、6.63倍(

0.05）；加入VEGF抗体100、50 μg的共培养组的MMP-2 mRNA相对表达倍数较共培养组分别下调了2.08、2.11倍（

0.05），蛋白表达下调了2.12、1.71倍（

0.05）；加入VEGF抗体100 μg的共培养组的MMP-9 mRNA相对表达倍数较共培养组下调2.13倍（

0.05），蛋白表达下调了2.94倍（

0.05）。结论 SKOV3可能通过分泌VEGF调节EPCs MMP-2、MMP-9蛋白的表达，从而促进EPCs的成管能力。

Abstract

Objective To observe the effect of vascular endothelial growth factor on the capacity of tube formation and expression of MMP-2 and MMP-

9 in endothelial progenitor cells (EPCs) after co-cultured with ovarian cancer cell line SKOV3. Methods Density gradient centrifugation was used to isolate EPCs. The capacity of tube formation was determined in EPCs that was cultured in Martigel (EPCs group) and co-cultured with SKOV3 cells (co-culture group)

respectively

and the tube formation capacity of EPCs was also determined after adding different concentrations of anti-VEGF antibody in the co-culture system. RT-qPCR and Western boltting were applied to determine the expression of MMP-2/MMP-9 in EPCs. Results The average number of tube formation in the EPCs group (18.00±1.00) was significantly lower than that in the co-culture group (36.33±1.52) (

0.05). The average numbers of tube formation in the co-culture group added with 25

50 and 100 μg of anti-VEGF antibody were 32.00±1.00

26.33±0.57 and 20.33±1.15

respectively

which were all less than that in the co-culture group without anti-VEGF antibody (

0.05). The results of RT-qPCR and Western blotting showed that the co-culture group showed an increase in the mRNA and protein expression of MMP-2 and MMP-9 as compared with the EPCs group by 4.36 fold (

0.05)

3.34 fold (

0.05) and 2.99 fold (

0.05)

and 6.63 fold (

0.05)

respectively. Adding with 100 and 50 μg anti-VEGF antibody into the co-culture group resulted in an inhibition in the mRNA and protein expression of MMP-2 by 2.08 fold (

0.05) and 2.11 fold

0.05)

and 2.12 fold (

0.05) and 1.71 fold (

0.05)

respectively. Adding 100 μg anti-VEGF antibody into the co-culture group resulted in an inhibition in the mRNA and proteins expression of MMP-9 by 2.13 fold (

0.05) and 2.94 fold (

0.05)

respectively. Conclusion SKOV3 cells may regulate the expression of MMP-2 and MMP-9 in EPCs by secreting VEGF

thereby promoting the tube formation capacity of EPCs.

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