Objective      To study the effect of stromal cell-derived factor-1 (SDF-1) on human bone marrow mesenchymal stem cells (BMSCs) differentiating into vascular endothelial cells (VECs).       Methods      G

roup of experiments: a blank group (A); experimental groups: groups of 10

25 and 50 ng/mL SDF-1(B1/B2/B3)

and group of 25 ng/mL VEGF+25 ng/mL SDF-1 (B4); and control groups: group of 25 and 50 ng/mL VEGF (C1/C2). The BMSCs of different groups were cultured in serum-free media with the corresponding cytokines for 12 d. Immunohistochemistry was applied to detect positive rates of CD31 and von Willebrand Factor(vWF) on the BMSCs. qPCR was applied to measure the mRNA levels of vWF

vascular cell adhesion molecule(VCAM-1)

vascular endothelial growth factor receptor-2(KDR)

VE-cadherin(VE) and CD31.       Results      The mRNA levels of KDR

VE

vWF

CD31 and VCAM-1 and positive rates of CD31 and vWF in the B4 group were significantly higher than those in the B1

B2 and B3 groups (

P

0.01). The mRNA levels of VE and vWF and positive rate of vWF in the B4 group were significantly higher than those in the C1 group (

P

0.01). The mRNA levels of KDR

CD31 and VCAM-1 and positive rate of CD31 in the B4 group were significantly higher than those in the C2 group (

P

0.01).       Conclusion      SDF-1 can promote BMSCs differentiating into VECs

and SDF-1 combined with VEGF is more effective than single use of VEGF or SDF-1.