Objective      To determine the biological effects of PCDH8 gene expression in nasopharyngeal carcinoma cell line CNE-1.       Methods      The eukaryotic expression plasmid pcDNA3.1-PCDH8 and pcDNA3.1-vector were transfected into CNE-1 cells

respectively. RT-PCR and Western blotting were used to examine the expression of PCDH8 at mRNA and protein levels after transfection. CCK-8 assay

flow cytometry

invasion assay

and chemosensitivity test were used to determine the

effects of PCDH8 expression on the proliferation

chemosensitivity

growth

and invasion in CNE-1 cells.       Results      RT-PCR and Western blotting showed that the PCDH8 expression was obviously higher in the cells transfected with pcDNA3.1-PCDH8 than those transfected with pcDNA3.1-vector. Cell growth assay by CCK-8 assay indicated that a significant reduction in cell proliferation in the cells transfected with pcDNA3.1-PCDH8 than the cells transfected with blank vector. Flow cytometry proved that exogenous PCDH8 expression resulted in a significant accumulation of cells at the G

0

/G

1

phase compared with the control cells

and a concomitant decrease of the cells at the S phase compared with control group cells. Transwell matrigel invasion assay revealed an obvious reduction in the invasive ability of the PCDH8 transfected cells than the control cells. The chemosensitivity assay showed exogenous PCDH8 expression significantly enhanced the chemosensitivity of NPC cells to cis-platinum compared with the control cells.       Conclusion      The PCDH8 eukaryotic expression vector decreases the proliferation

growth

and invasion ability

and increases the cis-platinum sensitivity in CNE-1 cells.