Objective        To sequence the Acinetobacter baumannii bacteriophage AB3 separated by our team

and to perform bioinformatics analysis

so as to identify the classification of its phylogenetic relationship.       Methods       Shot-gun library and config package strategy were carried out for sequencing the genome of bacteriophage AB3. Such software as EditSeq

tRNAscan-SE

TRF

FindTerm

ORF finder

BPROM and GeneMark

TM

were applied to predict both general characteristics of the bacteriophage AB3 genome and the coding gene function. In addition

the evolution of RNA polymerase gene system was analyzed with the software of Clustalx and phylip.       Results        The genome of bacteriophage AB3 was a

double-strand DNA with a full length of 31 185 bp

in which G+C mol% was 39.18% and 28 predicted genes

1 transcription terminator

and 4 possible promoter sequences were included.       Conclusion       Genetic analysis and RNA polymerase gene evolution analysis indicate that bacteriophage AB3 is similar to bacteriophage AB1

and both of them belong to phiKMV-like virus.