Aβ<sub>1-42</sub>抑制少突胶质前体细胞增殖与分化的实验研究

张茂; 詹晓黎; 陈兴书; 蔡其燕; 徐妍; 姚忠祥

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Aβ_1-42抑制少突胶质前体细胞增殖与分化的实验研究

论著 | 更新时间：2026-01-09

- Aβ_1-42抑制少突胶质前体细胞增殖与分化的实验研究
- Inhibitory effect of _1-42 on proliferation and differentiation in oligodendrocyte precursor cells
- 陆军军医大学学报 2013年35卷第12期页码：1183-1187
- 作者机构：
- 作者简介：
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- DOI：
  中图分类号： R329.28;R341;R745.702
- 纸质出版：2013
- 稿件说明：
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张茂,詹晓黎,陈兴书,蔡其燕,徐妍,姚忠祥.Aβ1-42抑制少突胶质前体细胞增殖与分化的实验研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(12):1183-1187.

　Zhang Mao,Zhan Xiaoli,Chen Xingshu,Cai Qiyan,Xu Yan,Yao Zhongxiang.Inhibitory effect of 1-42 on proliferation and differentiation in oligodendrocyte precursor cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(12):1183-1187.
张茂,詹晓黎,陈兴书,蔡其燕,徐妍,姚忠祥.Aβ1-42抑制少突胶质前体细胞增殖与分化的实验研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(12):1183-1187. DOI：

　Zhang Mao,Zhan Xiaoli,Chen Xingshu,Cai Qiyan,Xu Yan,Yao Zhongxiang.Inhibitory effect of 1-42 on proliferation and differentiation in oligodendrocyte precursor cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(12):1183-1187. DOI：

摘要

目的探讨在离体细胞培养条件下， Aβ

1-42

对SD乳鼠少突胶质前体细胞(oligodendrocyte precursor cells

OPCs)增殖与分化的影响。方法采用条件培养液原代培养获得高纯度SD乳鼠OPCs，分为正常组、加入无菌水的对照组、加入Aβ

1-42

的实验组，采用CCK-8检测存活细胞数量和增殖水平， Western blot 检测细胞凋亡蛋白含量，免疫细胞化学染色观察细胞形态变化及分化情况。结果与对照组相比，实验组OPCs存活率显著降低（

0.01），具体表现为：Aβ

1-42

作用OPCs 1 h后，细胞增殖明显受到抑制（

0.01）；药物浓度1 mol/L作用于OPCs时，增殖也受到影响（P0.05），当Aβ

1-42

剂量增加至2.5 mol/L时，细胞增殖明显受到抑制（

0.01），而对照组与正常组无显著差异（

0.05）。对凋亡蛋白Caspase-3的检测发现，它们在对照组与正常组的表达水平基本相当（

0.05），而在实验组明显升高（

0.01），且呈现时间和浓度依赖性，具体表现为：Aβ

1-42

作用OPCs 2 h后，Caspase-3表达水平明显上调（

0.01）；药物浓度2.5 mol/L作用于OPCs时，蛋白表达水平有所升高（

0.05），当Aβ

1-42

剂量增加至5 mol/L时，Caspase-3表达水平明显上调（

0.01）。对成熟少突胶质细胞标记物髓鞘碱性蛋白的免疫染色发现，与对照组相比，实验组的细胞体积明显变小，突起明显减少。结论 Aβ

1-42

可能通过促使细胞凋亡而抑制OPCs增殖，并抑制了OPCs向成熟少突胶质细胞分化，从而影响中枢神经系统的髓鞘形成。

Abstract

Objective To determine the effect of Aβ

1-42

on the biological behavior of cultured mouse oligodendrocyte precursor cells (OPCs). Methods Cultured and purified

mouse OPCs were divided into three groups including a normal group

a control group and an experimental group with Aβ

1-42

. The survival rate of OPCs was assessed by CCK-8 assay. The differentiation and morphology of OPCs were assessed by immunocytochemistry. The apoptosis of OPCs was assessed by Western blotting. Results As compared with the control group

the survival rate of OPCs in the experimental group significantly decreased (P0.01). Specifically

the proliferation of OPCs was dramatically suppressed by Aβ

1-42

in 1 h (

0.01)

and the suppression increased in a dose-dependent manner with the difference changed from

0.05 for Aβ

1-42

at 1 mol/L to P0.01 for it at 2.5 mol/L. However

there was no significant difference between the control group and normal group (

0.05). The level of caspase-3 in the experimental group was significantly higher than that in the control group and normal group (

0.01). Moreover

the expression of caspase-3 was significantly increased by Aβ

1-42

in a time- and dose-dependent manner (

0.05 for Aβ

1-42

at 2.5 mol/L

0.01 for Aβ

1-42

it at 5 mol/L). Compared with the control group

the diameter and processes of oligodendrocytes in the experimental group decreased significantly through detecting the myelin basic protein. Conclusion Aβ

1-42

can significantly suppress the proliferation of OPCs through inducing cell apoptosis

and inhibit OPCs differentiation into oligodendrocytes

thus affecting the myelination in central nervous system (CNS).

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Aβ1-42抑制少突胶质前体细胞增殖与分化的实验研究

Inhibitory effect of 1-42 on proliferation and differentiation in oligodendrocyte precursor cells

DOI：

摘要

Abstract

关键词

Keywords

references

Aβ_1-42抑制少突胶质前体细胞增殖与分化的实验研究

Inhibitory effect of _1-42 on proliferation and differentiation in oligodendrocyte precursor cells