Objective      To determine the concentrations of sodium and potassium in the dander from obese patients

and the expression of sodium- and potassium-related transporters at skin tissue

and to explore their clinical significance.       Methods      A total of 127 volunteers

75 females and 52 male with an average age of 52.83±6.11 (ranging from 40 to 60) were recruited from Chongqing region. The volunteers were divided into normal weight (n=50)

overweight (n=56) and obese group (n=21) by their body mass index (BMI)

or non

-abdominal obesity (n=81) and abdominal obesity group (n=46) by their waist circumference (WC). Dander scraped from medial forearm of subjects was digested by heated solution (1.5 ml 65% nitric acid and 0.5ml 70% perchloric acid)

then the concentration of sodium and potassium in the solution was detected by flame atomic spectrophotometry. Glucocorticoid-receptor

aldosterone receptor and Na

+

-K

+

-ATP enzyme in skin tissue samples from C57 mice and from human during operation were detected by Western blotting.       Results       At optimal reaction conditions

our method obtained a relative standard deviation ranging from 0.34 to 1.45 and a recovery rate ranging from 93.1% to 107.2%. Obese group had a significantly higher contents of sodium (P=0.002) and potassium (P=0.000)  when compared with normal weight group and overweight group. However

no significant difference was observed between normal weight group and overweight group. Western blotting showed that glucocorticoid-receptor

aldosterone receptor and Na

+

-K

+

-ATP enzyme were all expressed at mice and human skin tissue.       Conclusion      We establish the method to determine the dander contents of sodium and potassium from human skin tissue

and find increased concentrations of sodium and potassium in dander form obese subjects

suggesting that our method can easily reflect the tissue electrolyte change of obesity. Sodium and potassium transporter-related proteins are all expressed in mouse and human skin tissue.