Objective        To explore the expression profiles of multiple chemotherapy-related markers of DNA damage repair genes and thymidylate synthase (TS) β-tubulin Ⅲ in human osteosarcoma tissues and their relationship with clinical pathology of osteosarcoma.       Methods       Fifty cases of osteosarcoma tissue paraffin blocks were obtained from the patients with diagnosed osteosarcoma. Tissue microarray and immunohistochemical method were applied to detect gene expression levels of OGG1

BRCA1

RRM1

XRCC1

APE1

ERCC1

TS and β-tubulin Ⅲ in the osteosarcoma tissues.       Results        ①OGG1

BRCA1

RRM1

XRCC1

APE1 and ERCC1 were positively expressed in osteosarcoma tissues with the positive rates of 96%

94%

94%

84%

84% and 74%

respectively

and their strongly positive rates were 64%

76%

54%

72%

48% and 2%

respectively. There were only 6 cases showed weakly positive expression of TS

and β-tubulin Ⅲ was negative in osteosarcoma tissues. ②In the positively expressed genes

only APE1 expression was correlated with osteosarcoma pathological types (P0.05). ③The results of rank correlation analysis showed that APE1 expression was positively correlated with the expression of RRM1

OGG1 and XRCC1 (P0.05

P0.01)

OGG1 expression was positively correlated with BRCA1 expression (P0.05)

RRM1 expression was positively correlated with and XRCC1 expression (P0.01)

but the expression of BRCA1 was negatively correlated with ERCC1 expression (P0.05).       Conclusion        Several types of DNA damage repair genes are expressed in osteosarcoma tissues

and some of their expression levels have Spearman rank correlations. The characteristics of the chemotherapy-related gene expression in osteosarcoma may have significance for individual chemotherapy in patients with osteosarcoma.