Objective　　　 To investigate the role of transmembrane and ubiquitin-like domain-containing protein 1 (Tmub1) protein in the liver regeneration and its impact on the expression of securin in the process.　　　 Methods　　　 A total of 54 adult male SD rats were randomly divided into Tmub1 RNAi lentivirus group

blank lenitiviral vector group

and control

and they received an injection of lentivirus (3×10

7

TU) or PBS through ileocaecal vein. In 2 d later

partial hepatectomy (PH) were carried out in all rats. Then the liver tissue was collected in 0

2

6

12

24 and 48 h after model establishment to extract primary hepatocytes and preserve liver tissue. Effect of Tmub1 RNAi lentivirus interference on rat primary hepatocytes was detected by real-time PCR and Western blotting

respectively. Effect of Tmub1 RNAi lentivirus on the growth and cell cycle in rat primary hepatocytes was detected by MTT assay and flow cytometry

respectively.　　　 Results　　　 Real-time PCR and Western blotting showed that the expression of Tmub1 at mRNA and protein levels was significantly inhibited

and the expression of securin at mRNA level had no change

but the expression of securin at protein level was significantly down-regulated. MTT assay showed that Tmub1 gene silencing significantly improved the proliferation in liver cells in 6 to 24 h after PH. Flow cytometry displayed that the liver cells were arrested in G

2

/M phase.　　　 Conclusion　　　Tmub1 protein plays a negative role in the process of liver cells’ proliferation

which may be closely related to its effect on the expression of securin at protein level.