Objective       To investigate the effect of Y-27632

a selective inhibitor of ROCK-I

on the proliferation

apoptosis and adhesion of human ocular Tenon’s capsular fibroblasts (OTFs)  in vitro.       Methods        After induced by lysophosphatidic acid (LPA)

the fourth to sixth passages of OTFs were exposed to 6

30

150 and 750 μmol/L of Y-27632 for 48 h

respectively

and MTT assay was applied to determine the inhibition ratios of cell proliferation and adhesion. OTFs without LPA induction were treated with different concentrations of Y-27632

and Annexin V-FITC/PI double labeling flow cytometry was applied to determine cell apoptotic rate. The cell cloning efficiency was calculated by cell plate clonal expansion experiment.       Results        The proliferation inhibition D(490) and adhesion inhibition D(490) of OTFs treated with LPA+Y-27632 were significantly greater than those of LPA-induced OTFs (P0.05

P0.01). Cell apoptotic rate increased along with the increase of Y-27632 concentrations

and the cloning efficiency of OTFs without LPA induction decreased along with the increase of Y-27632 concentrations.       Conclusion       Y-27632 can effectively inhibit both proliferation and adhesion of LPA-induced OTFs

and induce early apoptosis of OTFs without LPA induction.