Objective      To determine the effect of blocking the PI3K/AKT pathway on the expression of CA916798 gene in nude mouse transplanted tumor of human lung adenocarcinoma cell line A549 and the multidrug-resistant cell line A549/CDDP.       Methods      A total of 20 nude mice were randomly and equally divided into 4 groups

that is

A549

A549/CDDP

A549+LY294002

and A549/CDDP+LY294002 groups. The animal model with A549 cells and A549/CDDP cells transplanted tumor was established in these corresponding nude mice. LY294002 of 25 mg/kg was treated in the mice of the 2 later groups. The size of tumor was measured

and morphology of the mass was observed after HE staining. Real-time fluorescent quantitative PCR was performed to detect CA916798 mRNA expression in the tumor.       Results      The model of transplanted tumor was established in nude mice  successfully. Blocking PI3K/AKT pathway with LY294002 resulted in significantly decreased expression of CA916798 at mRNA level (P0.05)

and reduced tumor volume.       Conclusion      Inhibition of PI3K/AKT pathway significantly inhibits the malignant proliferation in lung adenocarcinoma cell A549 and multidrug-resistant cell line A549/CDDP.