Objective        To establish a high throughput cell electro-fusion platform

by which to fuse mouse embryonic stem cells (mESCs) with somatic cells

and to explore the pluripotency of the fused cells.       Methods        With the microfluid chip developed by our lab

we fused the mESCs carrying GFP with NIH3T3 cells carrying RFP

and explored the aligment efficiency and electro-fusion efficiency. The fused cells were sorted by flow cytometery (FACS) and observed under a microscope. The mRNA levels of Nanog

Oct4

Sox2 and Lin28 in the three types of cells were detected by RT-PCR.       Results       A high throughput cell electro-fusion platform was developed. The aligment and electro-fusion efficiencies of mESCs and NIH3T3 fused cells were (44.35±10.99)% and (59.88±20.03)%

respectively. The fused cells can form embryonic stem cell-like clones. The RT-PCR results showed mESCs and fused cells rather than NIH3T3 cells could express Nanog

Oct4

Sox2 and Lin28.       Conclusion        Using the microfluid chip developed by our lab

the mESCs and NIH3T3 cells can be fused efficiently to reprogram somatic cells as pluripotent stem cells.