Objective      To construct a lentiviral vector co-expressing green fluorescent protein (GFP) and human phosphoglucose isomerase (PGI)

and to study the effect of human PGI gene interference on the proliferation of leukemia cells.       Methods       RT-PCR and Western blotting were used to detect the mRNA and protein expression levels of PGI in human mononuclear cells

K562 cells

KG1-α cells and HL-60 cells

respectively

to screen a leukemia cell line with high expression of PGI. Recombinant human PGI gene siRNA lentiviral vector and its negative control were constructed and packaged by 293T cells

and the lentiviral vector that could express PGI siRNA in mammal cells were obtained. Recombinant lentivirus (interference group)

original lentivirus (negative control group) and the same volume of phosphate buffer solution (PBS) (blank control group) were used to transfect KG1-α cells

respectively. After KG1-α cells with stable transfection were obtained

the mRNA and protein expression levels of PGI were detected by semi-quantitative RT-PCR and Western blotting

and cell proliferation was examined by CCK-8 assay.       Results       PGI was highly expressed in KG1-α cells

and KG1-α-siPGI cells with stable and low PGI expression were successfully constructed. Compared with the blank control group

the proliferation capability of KG1-α-siPGI cells decreased significantly (P0.05).       Conclusion       We successfully construct the KG1-α leukemia cell line with low expression of PGI

and the interference of PGI expression can inhibit the proliferation of KG1-α cells.