Objective      To investigate the difference of gene expression between activated IBP-deficient and wild T lymphocyte by oligonucleotide microarray in order to determine the role of IBP in T lymphocyte activation.       Methods      Total RNA was isolated from IBP-deficient and its parental Jurkat T cells with anti-CD3 and anti-CD28 mAb stimulation for 24 or 48 h

and synthesized into double-stranded cDNA that was then synthesized into biotin-labeled cRNA probe by in vitro transcription. The cRNA probes were separately hybridized with 22K Human Genome Array Chip

and the signals were scanned by the GeneArray Scanner. The results were analyzed by bioinformatics.       Results      In comparison with the expression profile of parental Jurkat T cells

anti-CD3 and anti-CD28 mAb stimulation for 24 or 48 h resulted in that 56 genes were found to have no change in the expression

17 genes were up-regulated and 39 genes down-regulated in activated IBP-deficient Jurkat T cells. According to Gene Ontology and Tree View analysis

these genes were involved in energy metabolism

cell cycle

transcription and apoptosis and so on.       Conclusion      IBP-deficient causes differential expression in many genes in Jurkat T cells.