HuH7细胞系CD13<sup>+</sup>CD133<sup>+</sup>HCC细胞分选及CSCs特性分析

金世龙; 黄中荣; 陈华; 余天雾; 曹洪; 龙运全; 周健; 李鹤; 苟毅; 廖娟

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HuH7细胞系CD13⁺CD133⁺HCC细胞分选及CSCs特性分析

论著 | 更新时间：2026-01-09

- HuH7细胞系CD13⁺CD133⁺HCC细胞分选及CSCs特性分析
- Sorting and cancer stem cell characteristic analysis of CD13⁺CD133⁺HCC cells in HuH7 cell line
- 陆军军医大学学报 2012年34卷第16期页码：1658-1662
- 作者机构：
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  中图分类号： R329-33;R730.23;R735.7
- 纸质出版：2012
- 稿件说明：
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金世龙,黄中荣,陈华,余天雾,曹洪,龙运全,周健,李鹤,苟毅,廖娟.HuH7细胞系CD13+CD133+HCC细胞分选及CSCs特性分析[J].陆军军医大学学报(原第三军医大学学报),2012,34(16):1658-1662.

　Jin Shilong,Huang Zhongrong,Chen Hua,Yu Tianwu,Cao Hong,Long Yunquan,Zhou Jian,Li He,Gou Yi,Liao Juan.Sorting and cancer stem cell characteristic analysis of CD13+CD133+HCC cells in HuH7 cell line[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(16):1658-1662.
金世龙,黄中荣,陈华,余天雾,曹洪,龙运全,周健,李鹤,苟毅,廖娟.HuH7细胞系CD13+CD133+HCC细胞分选及CSCs特性分析[J].陆军军医大学学报(原第三军医大学学报),2012,34(16):1658-1662. DOI：

　Jin Shilong,Huang Zhongrong,Chen Hua,Yu Tianwu,Cao Hong,Long Yunquan,Zhou Jian,Li He,Gou Yi,Liao Juan.Sorting and cancer stem cell characteristic analysis of CD13+CD133+HCC cells in HuH7 cell line[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(16):1658-1662. DOI：

摘要

目的探讨CD13、CD133双荧光标志流式细胞术（fluorescence activated cell sorter，FACS）分选人肝癌HuH7细胞系CD13

CD133

HCC细胞方法及癌干细胞（cancer stem cells，CSCs）特征。方法 CD13、CD133双标志，采用FACS从人肝癌HuH7细胞系分选出CD13

CD133

HCC、CD13

CD133

HCC、CD13

CD133

HCC、CD13

CD133

HCC等4种细胞亚群；通过CD13

CD133

HCC细胞生长曲线，细胞周期，形成肿瘤球和裸鼠体内成瘤能力，及对5-FU、吡柔比星化疗药物的敏感性研究，分析CD13

CD133

HCC细胞是否具有CSCs生物学特征。结果人肝癌HuH7细胞系CD13

CD133

HCC细胞占23.8%

3.1%分选为CD13

CD133

HCC细胞

78.45%的CD13

CD133

HCC细胞处于G

期；CD13

CD133

HCC细胞增殖明显快于其他3个细胞亚群；在裸鼠10

个CD13

CD133

HCC细胞就可以成瘤，而1.0×10

个CD13

CD133

HCC只有2只成瘤(2/5)，干细胞培养CD13

CD133

HCC细胞8~15 d形成肿瘤球；CD13

CD133

HCC细胞对5-FU和吡柔比星具有抵抗特性，而其他3个亚群较易于杀灭。结论 CD13、CD133双标志FACS从HuH7分选的CD13

CD133

HCC细胞具有CSCs特征，可能成为HCC治疗靶细胞。

Abstract

Objective To study the sorting of CD13

CD133

HCC cell fraction in HuH7 cell line by fluorescence activated cell sorter (FACS) based on the expression of both CD13 and CD133 markers

and to analyze the cancer stem cells (CSCs) characteristics of CD13

CD133

HCC cells. Methods The antibodies against CD13 and CD133 were used as CSCs marker to sort CD13

CD133

HCC cells

CD13

CD133

HCC cells

CD13

CD133

HCC cells and CD13

CD133

HCC cells from HuH7 cell line by FACS. The growth curve

phase of cell cycle

sphere formation ability in vitro

tumor formation ability in vivo and susceptibility to 5-FU and pirarubicin of the CD13

CD133

HCC cells were investigated for CSCs identification and characterization of the CD13

CD133

HCC cells. Results The CD13

CD133

HCC cells accounted for 23.8% in HuH7 cell line

and 3.1% of the CD13

CD133

HCC cells was sorted as CSC-like cells. There was 78.45% of the CD13

CD133

HCC cells existed in G

phase. The proliferating ability of the CD13

CD133

HCC cells was significantly stronger than that of the other cell subsets. The CD13

CD133

HCC cells could form a tumor in naked mice after 4 weeks with a number of 10

but 1.0×10

of the CD13

CD133

HCC cells were needed to form a tumor. The in vitro sphere formation of the CD13

CD133

HCC cells was observed after 8 to 15 d. The CD13

CD133

HCC cells had the chemo-resistant ability to 5-FU and pirarubicin

but the other cell subsets could be killed easily by these drugs. Conclusion The CD13

CD133

HCC cells sorted from HuH7 cell line by FACS bas

ed on the expression of both CD13 and CD133 markers have the characteristics of CSCs

and may be the therapeutic target for human hepatocellular carcinoma.

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HuH7细胞系CD13+CD133+HCC细胞分选及CSCs特性分析

Sorting and cancer stem cell characteristic analysis of CD13+CD133+HCC cells in HuH7 cell line

DOI：

摘要

Abstract

关键词

Keywords

references

HuH7细胞系CD13⁺CD133⁺HCC细胞分选及CSCs特性分析

Sorting and cancer stem cell characteristic analysis of CD13⁺CD133⁺HCC cells in HuH7 cell line