Objective        To investigate the correlation of the viral load quantitation of high-risk HPV and P16 expression in cervical intraepithelial neoplasia（CIN）Ⅰ patients and its prognosis value.       Methods        A total of 103 inpatients with CINⅠ diagnosed by immunohistochemistry analysis in our hea

lth center from May 2008 to Jan. 2010 were enrolled in this study. All of the patients were analyzed the viral load quantitation of high-risk HPV by hybrid capture Ⅱ (HC-2). The expression of P16 in the cervix tissue were detected by immunohistochemistry at the same time. All of the patients were followed up for 6

12

18 and 24 months after first diagnosis for the examination of cytology

colposcopy

and cervical re-biopsy if necessary. The final histological results obtained at the last follow-up were considered as the sequel of CINⅠ. The correlation of the quantitation of high-risk HPV and P16 expression was evaluated for prediction of the evolvement of CINⅠ.       Results        The positive rate of high-risk HPV and P16 expression in CINⅠ were 69.90% （72/103） and 38.83%（40/103） respectively. The expression of P16 was positively correlated with the quantitation of HR-HPV in CINⅠ（r

s

=0.568 8，P0.01）. In the last follow-up (24 months)

12 cases (11.65%

12/103) were deteriorated into lesions over CINⅡ. When P16 expression with the grade ≥6 was regarded as predicting indicator for the deterioration of CINⅠ

the sensitivity

specificity and negative predictive value（NPV）were 91.67%

96.70%

and 98.88%

respectively (P0.01）. Whereas these values were 58.33%

81.32%

and 93.67%

respectively（P0.01) when the quantitation of HR-HPV ≥ 100 RLU/CO regard as cut-off value.       Conclusion      Both of the expression of P16 and the quantitation of HR-HPV are closely correlation with the prognosis of CINⅠ

and they can be considered as predict indictors for CIN deterioration.