Objective       To investigate the effect of gastric bypass on FoxO1 expression in the liver of rats with type 2 diabetes

and to explore the mechanism for gastric bypass ameliorating type 2 diabetes.       Methods       Forty SD rats were randomly divided into a diabetes operation group (group A

n=10)

a diabetes sham operation group (group B

n=10)

a normal control group (group C

n=10) and a diabetes group (group D

n=10). The levels of fasting blood glucose (FBG)

fasting and postprandial insulin and glucagon-like peptide-1(GLP-1) were measured before operation and at 4

8

16 and 24 weeks after operation. All the rats were sacrificed after 6 months

and the expression of FoxO1 in rat livers was determined by immunohistochemistry and Western blotting.       Results       As for the group A

the level of FBG decreased from 20.2±2.1 mmol/L before operation to 10.5±2.5 mmol/L at 4 weeks and 7.7±1.1 mmol/L at 24 weeks after operation

respectively. The level of fasting insulin increased from 2.87±0.08 mU/L before operation to 2.91±0.08 mU/L at 4 weeks and 3.0±0.06 mU/L at 24 weeks after operation

respectively. The level of fasting GLP-1 increased from 8.7±0.9 pmol/L before operation to 13.2±1.0 pmol/L at 4 weeks and 23.5±0.2 pmol/L at 24 weeks after operation

respectively. The expression level of FoxO1 was significantly lower in the group A (18.4±1.2) than in the group B (37.6±0.5) and group D (35.8±0.4) (P0.05)

and the FoxO1 expression was positively correlated with the serum level of GLP-1 in the group A (r=-0.864

P0.05).       Conclusion       Gastric bypass can significantly reduce the levels of blood glucose and FoxO1 expression in the liver of rats with type 2 diabetes.