Objective      To study the effect of albendazole (ABZ) on glycolysis of human cisplatin-resistant ovarian cancer SKOV3/DDP cells.       Methods      Proliferation of SKOV3/DDP cells was detected by methyl thiazolyl tetrazolium (MTT) colorimetry after treatment with ABZ in vitro. SKOV3/DDP cells were divided into control group

IC

25

g

roup

IC

50

group

and IC

75

group according to the concentration of ABZ with an inhibition rate of 0%

25%

50%

and 75%. Each group was further divided into 12

24 and 36 h subgroups according to the action time of ABZ at a certain concentration. Activities of hexokinase (HK)

pyruvate kinase (PK) and lactate dehydrogenase (LDH) were detected by colorimetry and ELISA

respectively

and expression of Akt mRNA and Myc mRNA was detected by RT-PCR at the setting time points.       Results      ABZ inhibited the proliferation of SKOV3/DDP cells in a dose-dependent manner (P0.05) with an inhibition rate of 0%

25%

50% and 75% at the concentration of 0

1.43±0.21

8.64±0.74  and 52.48±3.99 μmol/L

respectively

in control group

IC

25

group

IC

50

group and IC75 group. The activities of LDH

HK

PK and the expression levels of Akt mRNA and Myc mRNA were significantly lower in IC

25

group

IC

50

group and IC

75

group than in control group（P0.05）.       Conclusion      ABZ can inhibit the activities of glycolytic enzymes and down-regulate the expression of glycolysis-related genes in vitro.