Hong Mei,Wang Dong,Zhang Yong,Bao Lili.Identification of active ingredients and determination of contents in Xiaozhen Zhiyang capsule[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(20):2078-2081.
Hong Mei,Wang Dong,Zhang Yong,Bao Lili.Identification of active ingredients and determination of contents in Xiaozhen Zhiyang capsule[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(20):2078-2081.DOI:
Objective To identify 2 active ingredients and determine the contents of ferulic acid in Xiaozhen Zhiyang capsules. Methods Thin layer chromatography (TLC) was used for qualitative identification of chebula Fructus
and Ephedrae Herbap. The content of Ferulic acid from Rhizoma Chuanxiong was determined by HPLC. Results The identification method was of high specificity with clear thin layer chromatography spots. The negative control did not impose any disturbance. In HPLC
the chromatographic peak of ferulic acid was well isolated from other peaks. There was a good linear relationship between the peak area and the sample size in a range of 0.053 8 to 0.322 8 μg(r=0.999 9). The average recovery of ferulic acid was 100.30%
RSD=0.96% (n=9). Conclusion The method is a simple and accurate assay of good reproducibility. lt can be used for the quality control of Xiaozhen Zhiyang capsules.