Objective      To study the possibility of a tissue-engineered sling constructed by using bone marrow mesenchymal stem cells (BMSCs) and urinary bladder matrix (UBM) for therapy the treating stress urinary incontinence (SUI).        Methods      The BMSCs were isolated

cultured and amplified by combining  density gradient centrifugation combined with adhering adherent culture method. The amplified BMSCs were identified by FACS. The UBM was loaded by the third generation of BMSCs for 7 days

and the morphological characteristics of cultured the BMSCs were observed by using light microscopy

  and electron microscopy. The biomechanics markers of the stem cell sling including maximum load

Young’s modulus and extensibility stretching were tested by using a universal mechanical tester. The simple UBM

UBM loaded by smooth muscle cells (SMCs) and polypropylene materials were served as controls.       Results      The isolated and cultured BMSCs were isolated and cultured fusiform

refractive

and proliferative

with adult stem cell characteristics. Flow cytometric analysis showed the cell-surface antigens for on the BMSCs were CD29 (95.3%)

CD45 (1.52%) and CD31 (1.27%). The BMSCs or SMCs loaded on the UBM were able to grow in normally. The stem cell BMSCs sling and the simple UBM showed significant decrease in the maximum load[(0.025±0.003 and 0.018±0.004)kN vs (0.030±0.003 and 0.143±0.0341)kN

P0.01]； and the Young’s modulus significant decreased [(170.820±61.735 and 103.624±52.779) MPa vs (490.730±80.969 and 900.100±192.402)MPa

P0.01] but significant increased in the extensibility stretching [(16.418±1.014 and 22.684±6.848) mm vs (9.498±1.384 and 2.975±0.401) mm

P0.01] as compared with the SMCs sling and polypropylene materials

respectively.        Conclusion      The rat BMSCs are successfully isolated

  and cultured. We successfully built a kind of stem cell sling by culturing BMSCs on UBM. This kind of BMSCs sling and simple UBM showed the greater extensibility compared with the SMCs sling and polypropylene materials.