前胃泌素、胃泌素、膜联蛋白2对胃癌CD44<sup>+</sup>细胞增殖和侵袭的影响

程晓荣; 杨爱军; 刘燕菲; 李双明; 向琳; 杨丽; 李敏

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前胃泌素、胃泌素、膜联蛋白2对胃癌CD44⁺细胞增殖和侵袭的影响

论著 | 更新时间：2026-01-09

- 前胃泌素、胃泌素、膜联蛋白2对胃癌CD44⁺细胞增殖和侵袭的影响
- Effects of progastrin, gastrin and annexin 2 on proliferation and invasion in CD44⁺gastric cancer cells
- 陆军军医大学学报 2012年34卷第21期页码：2171-2175
- 作者机构：
- 作者简介：
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  中图分类号： R73-37;R730.23;R735.2
- 纸质出版：2012
- 稿件说明：
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程晓荣,杨爱军,刘燕菲,李双明,向琳,杨丽,李敏.前胃泌素、胃泌素、膜联蛋白2对胃癌CD44+细胞增殖和侵袭的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(21):2171-2175.

　Cheng Xiaorong,Yang Aijun,Liu Yanfei,Li Shuangming,Xiang Lin,Yang Li,Li Min.Effects of progastrin, gastrin and annexin 2 on proliferation and invasion in CD44+ gastric cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(21):2171-2175.
程晓荣,杨爱军,刘燕菲,李双明,向琳,杨丽,李敏.前胃泌素、胃泌素、膜联蛋白2对胃癌CD44+细胞增殖和侵袭的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(21):2171-2175. DOI：

　Cheng Xiaorong,Yang Aijun,Liu Yanfei,Li Shuangming,Xiang Lin,Yang Li,Li Min.Effects of progastrin, gastrin and annexin 2 on proliferation and invasion in CD44+ gastric cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(21):2171-2175. DOI：

摘要

目的检测前胃泌素、胃泌素、膜联蛋白2在CD44

细胞的表达情况，研究三者对CD44

细胞生长和侵袭能力的影响。方法使用CD44免疫磁珠分选技术从胃癌BGC-823细胞中分选出CD44

细胞，流式细胞仪(FCM)和免疫荧光检测CD44

细胞中CD44的表达，以鉴定分选是否成功。FCM细胞周期分析和无血清培养

检测CD44

细胞的干细胞特性。流式细胞仪检测前胃泌素、胃泌素、膜联蛋白2在CD44

细胞、BGC-823细胞和CD44

细胞表达的差异。 MTT法和Boyden小室实验研究3者对CD44

细胞生长和侵袭能力的影响。结果流式细胞仪和细胞免疫荧光结果提示：CD44

细胞中CD44阳性率为（90.20±2.24）%

显著高于BGC-823细胞[（9.57±2.35）%，P0.01

]

；CD44

细胞组处于G

期的细胞为（71.1±5.6）%，显著高于BGC-823细胞组[（47.7±3.5）%，P0.05

]

；在无血清培养7 d后，CD44

细胞能够形成圆形克隆球，而CD44

细胞不能形成圆形克隆球；流式细胞仪显示前胃泌素、胃泌素、膜联蛋白2在CD44

细胞中高表达，明显高于在BGC-823细胞和CD44

细胞的表达水平(P0.01)；Pearson相关性分析显示：三者的表达率呈正相关关系(P0.01)。高浓度的前胃泌素mAb和膜联蛋白2mAb能够抑制CD44

细胞的生长和侵袭，与对照组相比差异显著（P0.05）。胃泌素mAb不能抑制CD44

细胞的生长和侵袭，与对照组相比差异不显著（P0.05）。结论 CD44

细胞中高表达前胃泌素、胃泌素、膜联蛋白2，并且前胃泌素mAb和膜联蛋白2mAb能够抑制CD44

细胞的生长和侵袭能力，提示前胃泌素与膜联蛋白2能够促进CD44

细胞的生长和侵袭。

Abstract

Objective To investigate t

he expression of progastrin

annexin 2

and gastrin in CD44

gastric cancer cells

and determine their effects on the proliferation and invasion in the CD44

cells. Methods CD44 immunomagnetic beads were used to sort CD44

cells from gastric cell line BGC-823

and the sorted cells were identified by flow cytometry and cell immunofluorescence for the expression of CD44. Flow cytometry-cycle analysis and serum-free culture methods were used to examine CD44

cells’ characteristics of stem cells. The expression of progastrin

gastrin

and annexin 2 in CD44

cells

BGC-823 cells and CD44

cells were detected by flow cytometry

cell immunofluorescence and immunohistochemistry assays. The effect of them on the proliferation and invasion in the CD44

cells were detected by MTT assay and Boyden chamber. Results Flow cytometry and cell immunofluorescence assay showed the expression of CD44 in CD44

cells [(90.20±2.24)%

]

was markedly higher than in BGC-823 cells [(9.57±2.35)%

P0.01

]

. There were (71.1±5.6)% CD44

cells arrested in G

phase

which was obviously higher than that in BGC-823 cells [(47.7±3.5)%

P0.05

]

. The CD44

cells formed circular clone balls under serum free culture for 7 d

while the CD44

cells could not. The expression of progastrin

gastrin and annexin 2 in CD44

cells was significantly higher than in BGC-823 cells and CD44

cells (P0.01). Pearson correlation analysis showed that there was a positive correlation among their expression (P0.01). High concentrations of progastrin mAb and annexin2 mAb inhibited the proliferation and invasion of CD44 cells

and there was statistical significance compared with control group (P0.05)

but gastrin mAb could not (P0.05). Conclusion CD44

cells widely express progastrin

gastrin

and annexin 2. Progastrin mAb and annexin2 mAb inhibit the proliferation and inva

sion

which suggest that these two may promote cell proliferation and invasion.

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前胃泌素、胃泌素、膜联蛋白2对胃癌CD44+细胞增殖和侵袭的影响

Effects of progastrin, gastrin and annexin 2 on proliferation and invasion in CD44+ gastric cancer cells

DOI：

摘要

Abstract

关键词

Keywords

references

前胃泌素、胃泌素、膜联蛋白2对胃癌CD44⁺细胞增殖和侵袭的影响

Effects of progastrin, gastrin and annexin 2 on proliferation and invasion in CD44⁺gastric cancer cells