Objective      To determine the activation of the farnesoid X receptor (FXR) on the expression of fibroblast growth factor 21 (FGF21) in C57BL/6 mice.       Methods      A total of 18 male C57BL/6 mice (3 to 4 weeks old) were randomly divided into 3 groups and treated with FXR agonist

chenodesoxycholic acid (CDCA) at different doses of 0

10

and 50 mg/kg respectively for a week. After the treatment

the mice was sacrificed for their liver tissue. RT-PCR and Western blotting were performed to test the expression of FGF21 at mRNA and protein levels in the liver tissue. Blood triglycerides (TG)

cholesterol (TC) and blood glucose (GLU) were measured by enzymatic determination test.        Results      FXR agonist CDCA up-regulated the FGF21 at mRNA and protein levels in a dose-dependent manner in C57BL/6 liver tissue after treatment for a week. While

blood concentrations of TG

TC and GLU were decreased with the increase of CDCA doses.       Conclusion      CDCA up-regulates FGF21 expression in C57/BL6 mice liver and results in a decrease in plasma TG

TC and blood glucose.