Objective      To investigate the effect of hsa-miR-200a on the proliferation and apoptosis in human endometrium adenocarcinoma cell line HEC-1B and the potential mechanisms.       Methods      The expression of hsa-miR-200a in HEC-1B cell line was measured by real-time fluorescence quantitative PCR (FQ-PCR). The hsa-miR-200a mimics and inhibitor were synthesized and transferred into HEC-1B cell line by lipofectamine

respectively

and the cell proliferation and apoptosis were detected by methyl-thiazolyl-tetrazolium (MTT) assay and flow cytometry (FCM). Bioinformatics analysis was used to predict the target genes of has-miR-200a on cell proliferation and apoptosis. Western blotting and FQ-PCR were used to determine the protein and mRNA expression of phosphatase and tensin homolog deleted on chromosome ten (PTEN).       Results      Hsa-miR-200a expression was observed in HEC-1B cells. The hsa-miR-200a mimics and inhibitor were successfully transferred into HEC-1B cells

and the expression of hsa-miR-200a was significantly changed after the transfection. Compared with those of the blank control group and negative control group

HEC-1B cell proliferation was significantly inhibited in the miR-200a inhibitor group. The FCM results showed that the apoptotic rate increased in the hsa-miR-200a inhibitor group. The bioinformatics analysis results showed that SON

Pdcd4 and PTEN were the target genes of has-miR-200a. PTEN protein expression significantly decreased in the HEC-1B cells transfected with hsa-miR-200a mimics

and significantly increased in the HEC-1B cells transfected with hsa-miR-200a inhibitor. SON and Pdcd4 protein expression did not change before and after transfection.       Conclusion      Hsa-miR-200a is negatively correlated with PTEN protein expression in HEC-1B cell line

indicating that hsa-miR-200a may affect the proliferation and apoptosis in HEC-1B cells by regulating PTEN protein expression.