Objective        To study the effect of ileal transposition (IT) on lipid metabolism in liver of goto-kakizaki (GK) rats.       Methods        Twenty GK rats were randomly divided into operation group and sham operation group according to the random digital representation method. Rats in operation group underwent IT and those in sham operation group underwent in situ anastomosis of the distal ileal after transection. Levels of fasting and 2 h OGTT glucose were measured before and 1

2

4 weeks after operation

and levels of fasting glucagon-like-peptide-1 (GLP-1)

free fatty acids (FFA)

total cholesterol (Tch) and triglyceride(TG) were measured 4 weeks before and after operation. Liver tissue samples were taken 4 weeks before and after operation and stained with oil red O. TG sedimentation in liver was observed and its average gray-scale value was calculated with an image and graphic processor.       Results        The fasting and 2 h OGTT glucose levels were significantly lower in operation group 2 and 4 weeks after operation than before operation (P0.05) with no significant difference observed in sham operation group. The GLP-1 level was significantly higher while the FFA level was significantly lower in operation group in 4 weeks after operation than before operation (P0.05). However

no significant difference was observed in TG and Tch level between the two groups before and after operation (P0.05). The TG sedimentation in liver tissue samples was significantly less in operation group 4 weeks after operation than before operation with no significant change observed in sham operation group (P0.05).       Conclusion        IT can significantly reduce the blood glucose level in GK rats by improving the postoperative lipid metabolism in liver

and increasing GLP-1 secretion and alleviating insulin resistance due to the early arrival of food to the terminal ileum.